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Sequences of primer pairs encoding different target genes for semiquantitative RT-PCR*

Target Gene   Primer Sequence   Orientation   Position   Length (bp)   Annealing Temp (°C)   Reference   S-16   5'-TCCGCTGCAGTCCGTTCAAGTCTT-3'   Sense   15-38   385   ...   Chan et al, 1990     5'-GCCAAACTTCTTGGATTCGCAGCG-3'   Antisense   376-399         ß-Actin   5'-TCACCGAGGCCCCTCTGAACCCTA-3'   Sense   314-337   641   ...   Nudel et al, 1983     5'-GGCAGTAATCTCCTTCTGCATCCT-3'   Antisense   931-954         Axin   5'-AGTTTCACCGAAGATGCC-3'   Sense   58-75   624   50   Ikeda et al, 1998     5'-ACTGCAGACCTTTGGACT-3'   Antisense   664-681         Zyxin   5'-CATCACTGACCGAATGCC-3'   Sense   6-23   304   52   Cattaruzza et al, 2002     5'-AGAGTGGCACTTCCGACA-3'   Antisense   292-309         WASP   5'-TGTGACTATGTCTTCAGC-3'   Sense   105-122   438   48   Fukuoka et al, 1997     5'-TGGGAGATGTTGTTGACT-3'   Antisense   525-542         c-Kit receptor   5'-ATCAGGCTGACGTGCACT-3'   Sense   160-177   244   49   Tsujimura et al, 1991     5'-GGACCAGTGCGTCGTTGT-3'   Antisense   386-403         Fibronectin   5'-ATCAGCAGTGGGAACGGA-3'   Sense   188-205   223   51   Schwarzbauer et al, 1987     5'-GTACAGCTGATCCTGCCT-3'   Antisense   393-410         3ß-HSD   5'-AGGATCGTCCAGTTGTTG-3'   Sense   52-69   404   46   Simard et al, 1993     5'-GACCATGTGCTTTCATGA-3'   Antisense   438-455         Testin   5'-GCAGACATCCCAGGTGTATGAGACATGATTGCT-3'   Sense   -72--40   1053   61   Grima et al, 1995     5'-ACTCCTTCCTTGTCCCGGGGCAGTAGCTCATAC-3'   Antisense   949-981         *The annealing temperature used for S-16 or ß-actin in each RT-PCR experiment for coamplification was the same as the target gene, ranging from 46°C to 61°C.   3ß-HSD indicates 3ß-hydroxysteroid dehydrogenase; RT-PCR, reverse transcriptase-polymerase chain reation; and WASP, Wiskott-Aldrich syndrome protein.

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