Figure 6. (A) Reverse transcription polymerase chain reaction (RT-PCR) using mRNA isolated from the prostate of control and castrated rats at different stages after castration. Smoothelin mRNA was found even 21 days after castration. (B) Amplification of the ß-actin mRNA. Ct indicates control; 7d, 14d, and 21d, 7, 14, and 21 days after castration, respectively; Lu, lung; PC3, PC3 prostatic epithelial cancer cells; 1 and 2, two mRNA for smoothelin corresponding to the visceral and vascular isoforms, respectively. Key molecular markers are indicated on the right.