Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
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Table 6. List of 23 genes showing differential expression pattern as identified by microarray analysis and quantitative polymerase chain reaction*
          Reported Testis Expression     Fold Change in QPCR
 
    Gene Description   GenBank ID   Unigene ID   Biological Function   SgA   PcSc   RdSd   CT in RdSd{dagger}

  Group I   Glucokinase activity, related sequence 1 (Gk-rsl)   AI449806   Mm.32242   Yes{ddagger}   Unknown   0   0.78 ± 0.05   1§   24.62 ± 0.50
    Smafl   AI413624   Mm.28800   Yes   Unknown   0.03 ± 0.004   0.42 ± 0.02   1   27.84 ± 0.11
    Complement receptor 2 (Cr2)   AI451901   Mm.1226   No   Signaling   0.14 ± 0.02   0.27 ± 0.05   1   33.61 ± 0.08
    T-cell receptor CD3 eta chain precursor (Cd3{eta})   AI429824   Mm.1224   No   Signaling   0.28 ± 0.01   0.63 ± 0.01   1   28.41 ± 0.05
    EST weakly similar to B24264 proline-rich protein MP3 (fragment)   AI415354   Mm.156164   Yes   Unknown   0   0.42 ± 0.01   1   32.55 ± 0.14
    Hypothetical protein MNCb-2040   AI448690   Mm.142843   Yes   Unknown   0.06 ± 0.01   0.11 ± 0.002   1   22.30 ± 0.02
    EST weakly similar to human hypothetical protein FLJ20721   AI451034   Mm.34092   Yes   Unknown   0.06 ± 0.01   0.31 ± 0.01   1   25.68 ± 0.04
  Group II   G elongation factor (Gfm)   AI448386   Mm.27288   Yes   Protein biosynthesis   0.33 ± 0.05   3.03 ± 0.07   1   30.99 ± 0.22
    Regulator of G-protein signaling 2 (Rgs2)   AI464441   Mm.28262   Yes   Signaling   0.55 ± 0.05   1.27 ± 0.14   1   35.27 ± 0.28
    Potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4)   AI447526   Mm.195952   Yes   Signaling   1.22 ± 0.06   3.09 ± 0.07   1   31.97 ± 0.04
    EST weakly similar to 156134 human tumor necrosis factor alpha-induced protein 2   AI414503   Mm.27281   Yes   Unknown   0.05 ± 0.004   2.33 ± 0.05   1   26.91 ± 0.04
    EST weakly similar to myosin heavy chain, nonmuscle type B   AI465389   Mm.200636   Yes   Unknown   0.21 ± 0.02   4.50 ± 0.21   1   31.43 ± 0.12
    EST   AI449427   Mm.223650   No   Unknown   0.76 ± 0.04   7.36 ± 0.21   1   33.84 ± 0.55
    ESTs weakly similar to zinc finger protein 111   AI450088   Mm.30519   No   Unknown   1.25 ± 0.11   2.88 ± 0.14   1   33.13 ± 0.10
    EST highly similar to human nucleoprotein TPR   AI448719   Mm.210529   Yes   Nuclear protein import (?)   1.25 ± 0.09   3.32 ± 0.06   1   27.89 ± 0.15
  Group III   Tripartite motif protein 10 (Trim10)   AI662233   Mm.20159   No   Cell differentiation   1.89 ± 0.32   2.07 ± 0.49   1   34.61 ± 0.35
    EST weakly similar to PHD finger protein 1 (T-complex testis-expressed 3)   AI447598   Mm.21284   Yes   Unknown   1.64 ± 0.21   2.06 ± 0.20   1   31.20 ± 0.09
  Group IV   Cartilage-associated protein (Crtap)   AI448820   Mm.20904   Yes   Cell differentiation   1.38 ± 0.02   0.48 ± 0.02   1   26.20 ± 0.04
    B-cell receptor-associated protein 31 (Bap31)   AI323595   Mm.17   Yes   Apoptosis   1.49 ± 0.05   0.22 ± 0.01   1   26.72 ± 0.02
    Centrin 2 (Cetn2)   AI326150   Mm.24643   Yes   Centriole duplication cytokinesis   1.89 ± 0.18   0.38 ± 0.01   1   31.49 ± 0.06
    Prolyl oligopeptidase (Pop)   AI429335   Mm.6735   Yes   Cell differentiation sperm motility   4.14 ± 0.28   0.47 ± 0.02   1   27.39 ± 0.05
    RIKEN cDNA 1110051B16   AI428924   Mm.23498   No   Unknown   4.24 ± 0.12   0.43 ± 0.07   1   36.99 ± 0.25
  Group V   EST   AI426564   No Unigene ID   No   Unknown   10.28 ± 0.89   2.34 ± 0.36   1   36.18 ± 0.57

  *QPCR indicates quantitative polymerase chain reaction; SgA, type A spermatogonia; PcSc, pachytene spermatocytes; RdSd, round spermatids; CT, threshold cycle; and EST, expressed sequence tag.
  {dagger}CT values of RdSd when expression level of RdSd is considered to be 1.
  {ddagger}Shown to be present in both spermatocytes and spermatids.
  §Expression level in RdSd is set to be 1 for comparison.





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