| |
Fresh Frozen, Mean ± SE |
Frozen After 24 h, Mean ± SE |
P |
|
| TUNEL |
|
|
|
| Bright* |
136 ± 29 |
213 ± 28 |
.07 |
Light |
292 ± 48 |
370 ± 27 |
.15 |
Dark |
1572 ± 75 |
1417 ± 48 |
.07 |
| Comet |
|
|
|
| DNA in tail, % |
38.1 ± 5.1 |
29.3 ± 4.3 |
.037 |
Tail moment |
36.0 ± 5.4 |
27.8 ± 6.8 |
.006 |
| Tail length, µm |
175.0 ± 15.5 |
152.2 ± 17.6 |
.033 |
|
| *Number of cells with high concentration of TUNEL-positive signal within the
sperm head. |
| Number of cells with low concentration of TUNEL-positive signal within
the sperm head. |
| Number of cells with no TUNEL-positive signal within the sperm head. |
| Product of the migration distance and normalized intensity, integrated
over the tail length. |
|
