| Enzyme |
Protease Inhibitor |
Hydrolysis Products (cpm) |
|
No enzyme |
... |
275 ± 28 |
Collagenase, 10 µg (total counts) |
... |
5750 ± 250 |
| Collagenase, 0.5 µg |
... |
3470 ± 180 |
| Collagenase, 0.5 µg |
STI, 10 µg |
3320 ± 220 |
| Collagenase, 0.5 µg |
TIMP-1, 5 µg |
480 ± 33 |
| Collagenase, 1.5 µg |
TIMP-1, 5 µg |
760 ± 46 |
|
| *Assays were performed as described in "Materials and Methods,"
using highly purified TIMP-1 isolated from SCCM. Each number represents the
mean ± SD of triplicate wells from an experiment. This experiment was
repeated 3 times using different batches of TIMP-1, and similar results were
obtained each time. Data were not pooled since the amount of
[125I]-collagen coated per well in each experiment varied, yielding
different total counts. |
| Nonspecific control represent counts, ie, [125I]-collagen,
that were released nonspecifically from coated wells. |
| Total counts represents hydrolyzed fragments of
[125I]-collagen caused by collagenase-induced proteolysis. |
|
