|
|
GAL 1-lacZ Expression (Miller units)
|
| GBD Fusion |
GAD Fusion |
Acetate |
Glucose |
|
| ... |
... |
0.48 ± 0.04 |
0.03 ± 0.001 |
| Rim11p |
... |
42.9 ± 1.5 |
0.02 ± 0.004 |
| mGSK-3ß |
... |
0.24 ± 0.05 |
0.02 ± 0.009 |
| ... |
Ume6p (1-161) |
0.39 ± 0.05 |
0.02 ± 0.01 |
| Rim11p |
Ume6p (1-161) |
318.3 ± 102.0 |
0.23 ± 0.14 |
| mGSK-3ß |
Ume6p (1-161) |
86.3 ± 46.6 |
0.09 ± 0.006 |
| ... |
Ume6p-T99N (1-161) |
0.39 ± 0.02 |
0.02 ± 0.002 |
| Rim11p |
Ume6p-T99N (1-161) |
33.4 ± 10.1 |
0.02 ± 0.005 |
| mGSK-3ß |
Ume6p-T99N (1-161) |
0.81 ± 0.04 |
0.01 ± 0.006 |
| ... |
Ume6p-Ala5 (1-161) |
0.35 ± 0.05 |
0.04 ± 0.02 |
| Rim11p |
Ume6p-Ala5 (1-161) |
21.9 ± 2.1 |
0.02 ± 0.005 |
| mGSK-3ß |
Ume6p-Ala5 (1-161) |
0.43 ± 0.21 |
0.02 ± 0.009 |
|
| *GAD indicates GAL4 activation domain; GBD, GAL4 DNA-binding
domain; GSK-3ß, glycogen synthase kinase-3ß; mGSK-3ß, muring
GSK-3ß; Rim 11p, regulator of inducer of meiosis; SC, synthetic complete;
and Ume6p, unscheduled meiotic gene expression. |
 GAD fusions were transformed into strain Y187 and selected on SC-Leu
plates. GBD fusions were transformed into Y190 and selected on SC-Trp plates.
Strains were mated on yeast peptone dextrose plates, and diploids were
selected on SC-Trp-Leu plates. The values were means ± standard
deviations from 3 independent transformants. |
|
