Figure 6. (A) Linear regression analyses between percentages of DNA fragmented sperm (at 0, 4, and 24 hours of incubation) and percentages of necrotic sperm at the corresponding time points (time zero: solid line, r = 0.72, SD = 5.9, n = 14, P < 0.01; 4 hours: dashed line, r = 0.74, SD = 6.7, n = 14, P < 0.01; 24 hours: dotted line, r = 0.89, SD = 6.4, n = 14, P < 0.001). (B) Linear regression analysis between percentages of basal Ann V⁺/PI^– spermatozoa and the corresponding percentage rates of de novo (24 hours) sperm necrosis (n = 16). (C) Linear regression analysis between percentages of basal Ann V⁺/PI^– spermatozoa and the corresponding percentage rates of de novo (24 hours) sperm DNA fragmentation (n = 14).