Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
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Table 2. Ligand binding properties of normal and mutant ARs stably expressed in PC-3 cells
Expression Plasmid Mutation Hormone Bmax (fmol/mg protein) Apparent Kd (nM)
CMV-AR None 3[H]-DHT 63.38 0.8
CMV-AR-Q20-V108K V108K 3[H]-DHT 271.3 0.9
CMV-AR-Q26-V108K V108K 26 glutamines in the poly-Q repeat 3[H]-DHT 119.77 0.9





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