Figure 7. RT-PCR analyses of GC3, GC12, GC14, and ubiquitin from specific tissues. RT-PCR products (10 µL) were separated on a 1% agarose/0.5% TAE gel followed by ethidium bromide staining. Total RNA was isolated from spleen, skeletal muscle (Skel. Mus.), brain, kidney, heart, liver, lung, and testes (testis) of 40-day-old mice for RT-PCR analyses. -cDNA indicates that cDNA was replaced with water.