Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
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Table 5. Comparison of oxidative stress indices (ROS, TAC, and ROS-TAC score) between donors, group 1 (smokers with a normal genital examination) and group 2 (nonsmokers with a normal genital examination)
Variable Donors (n = 13) Group 1 (n = 12) Group 2 (n = 21) A B C

ROS (x 106 cpm) 0.1 (0.09, 0.65) 63 (6.1, 451) 3.2 (0.7, 13) 0.02 0.03 0.04
TAC (Trolox Equivalent) 985 (825, 1755) 563 (409, 814) 1003 (901, 1251) 0.02 0.85 0.01
ROS-TAC Score 53 (51, 63) 33 (29, 39) 50 (45, 55) 0.005 0.06 0.01
Seminal Leukocytes (x 106/mL) 0.1 (0, 0.2) 1.8 (0.6, 4.4) 0.0 (0.0, 0.4) 0.006 0.2 0.01

Values are median (25th, 75th percentiles). ROS = reactive oxygen species in washed sperm suspensions (after simple wash and resuspension in PBS) measured by chemiluminescence assay. TAC = total antioxidant capacity in seminal plasma measured by enhanced chemiluminescence assay. A = P value of donors versus group 1. B = P value of donors versus group 2. C = P value of group 1 versus group 2. Wilcoxon rank-sum test was used for the analysis and P < .05 was significant.





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