Figure 6. Changes in AR and SDH levels in testes around prepubertal and postpubertal stages. (a) Enzymatic activities of 90 µg of cytosolic proteins from prepubertal and postpubertal rat testes were measured using MG and sorbitol as substrates. Data presented are means + SDs for 3 rat organs. (b) Ten micrograms of protein from testis were subjected to Western blot analysis with a 1:3000 dilution of AR (left panel) and a 1:3000 dilution of SDH (right panel) antibodies. (c) Total RNAs (5 µg) from rat testes at the indicated ages were separated on 1% agarose gel. The blotted membrane was hybridized with the AR probe (left panel) and, after stripping, with SDH probe (right panel), consecutively. The 18S and 28S ribosomal RNAs are shown after staining the agarose gel with ethidium bromide (lower left panel).