Figure 3. Immunocytochemical detection of DAZAP1 (Deleted in Azoospermia Associated Protein 1) in mouse testes. Testicular sections were prepared from adult (a-m) and prepubertal mice at day 10 (n) and day 18 (o). Immunostaining was carried out with the anti-DAZAP1 antibody (a, d-o), the preimmune antiserum (b), or the anti-DAZAP1 antibody preincubated with the oligopeptide antigen (c). No counterstaining was applied to sections (f), (h), or (j). The stages of the tubules in (d-m) are indicated. The cells are B, type B spermatogonia; D, diplotene spermatocytes; E, elongated spermatids; M, meiotic cells; P, pachytene spermatocytes; R, round spermatids; and 2S, secondary spermatocytes. Bar = 0.2 mm in (a), 0.1 mm in (b, c), and 30 µm in (d-m).