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Figure 4. Effect of aging on peripheral benzodiazepine receptor (PBR) protein
expression. Whole cells (or mitochondria) were solubilized in Laemmli buffer.
Equal amounts of protein were loaded per lane and analyzed by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by immunoblot
detection of PBR using a rabbit anti-PBR antiserum. Blots were reprobed for
glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control.
Positive bands were detected by chemiluminescence and quantified by
densitometric analysis as described in "Materials and Methods."
Left: immunoreactive bands of PBR and GAPDH in samples from a representative
experiment. Right: histogram showing the relative densitometric units
expressed as percentages of the young samples and representing mean plus or
minus standard error of the mean of 4 independent experiments. Significantly
different from young value: ***P less than.001.
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