Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
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Table 2. Centroid motility parameter values (±SD, n = no. of sperm analyzed) for sperm subpopulations identified from PATN analysis*{dagger}
VCL, µm/s VAP, µm/s VSL, µm/s BCF, Hz ALH, µm LIN, % n (% of population)

Group 1 143.0 ± 20.7 83.4 ± 17.4 61.4 ± 33.9 12.3 ± 5.6 7.5 ± 3.2 44.6 ± 25.0 16,022 (62.3)
Group 2 90.6 ± 21.7 43.8 ± 19.1 20.7 ± 14.8 5.1 ± 5.2 8.0 ± 8.1 24.0 ± 16.5 8,824 (34.3)
Group 3 118.4 ± 30.9 48.7 ± 12.5 15.7 ± 4.8 84.6 ± 27.1 1.9 ± 0.7 14.3 ± 7.3 867 (3.4)
Mean 126.7 ± 30.4 73.7 ± 37.9 53.6 ± 38.4 13.1 ± 16.6 7.2 ± 7.6 41.5 ± 27.6 25,713 (100)

*VCL indicates curvilinear velocity; VAP, average path velocity; VSL, straight-line velocity; BCF, beat cross frequency; ALH, amplitude of lateral head displacement; and LIN, linearity.
{dagger}These particular data pertain to the total population of analyzed cells (ali incubations, all time points) in the study testing bicarbonate stimulation of sperm motility in 7 different ejaculates, of which absolute motility parameter values are presented in Figure 1 and Table 1. Changes in group 1 sperm (designated "fast linear"—compare VSL and LIN values) in these experiments are shown in Figure 3. The group 3 sperm, which differ markedly from the other types with respect to BCF, occurred only in a single sample (Figure 3h) and were active sperm that had stuck to the slide. Clear recognition of the small population of group 3 sperm illustrates the power of PATN analysis.





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