| RT-PCR Product
|
Size (bp)
|
IDV
|
% (based on IDV)
|
% Difference Between Young and Old Rats
|
|
ET-1 (Y)
|
378
|
68924
|
24.4
|
|
ET-1 (O)
|
378
|
58360
|
20.7
|
15.16
|
| eNOS (Y)
|
432
|
46515
|
16.5
|
|
| eNOS (O)
|
432
|
40705
|
14.4
|
12.73
|
| TGF-ß1 (Y)
|
278
|
26327
|
8.9
|
|
| TGF-ß1 (O)
|
278
|
24241
|
8.2
|
7.87
|
| VEGF-188 (Y)
|
564
|
24764
|
8.3
|
|
| VEGF-188 (O)
|
564
|
22440
|
7.6
|
8.43
|
| VEGF-164 (Y)
|
492
|
39353
|
13.3
|
|
| VEGF-164 (O)
|
492
|
31049
|
10.5
|
21.05
|
| VEGF-144 (Y)
|
432
|
24380
|
8.2
|
|
| VEGF-144 (O)
|
432
|
22147
|
7.5
|
8.54
|
| VEGF-120 (Y)
|
360
|
27647
|
9.3
|
|
| VEGF-120 (O)
|
360
|
23357
|
7.9
|
15.05
|
|
|
| *eNOS indicates endothelial nitric oxide synthase; ET-1,
endothelin-1; IDV, integrated density value; RT-PCR, reverse
transcription-polymerase chain reaction; TGF, transforming growth factor; and
VEGF, vascular endothelial growth factor.
|
| Y = young rat.
|
| O = old rat.
|
|
