Journal of Andrology
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Published-Ahead-of-Print June 10, 2010, DOI:10.2164/jandrol.110.010231

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Sperm Proteomics: Thinking Outside the Collision Cell

Senga K Oxenham *

* To whom correspondence should be addressed. E-mail: s.oxenham{at}dundee.ac.uk.

Many factors exist to make the study of proteins challenging at best. In sperm biology these are coupled with the fact that a single human ejaculate contains over 100 million sperm cells at different stages of development and in various states of activity. In addition, these cells are transported in an active carrier and exchange extensively with both male and female reproductive tracts; ultimately stochastic noise means defining function and malfunction of sperm proteins is a huge task. To make substantial progress in studying low level, fast changing, highly regulated and hence important proteins we must think outside the mass-spec instrument.


Key words: Infertility • Sperm • Bioinformatics • Proteomics






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