Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published-Ahead-of-Print June 10, 2010, DOI:10.2164/jandrol.109.009183

This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
32/1/86    most recent
Author Manuscript (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SELLAMI, H.
Right arrow Articles by HAMMAMI, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SELLAMI, H.
Right arrow Articles by HAMMAMI, A.

A Proposed Mouse Model to Study Male Infertility Provoked by Genital Serovar E, Chlamydia trachomatis

Hanen SELLAMI , Radhouane GDOURA *, Imed MABROUK , Olfa FRIKHA-GARGOURI , Leila KESKES , Zohair MALLEK , Mahjoub AOUNI , and Adnane HAMMAMI

* To whom correspondence should be addressed. E-mail: gdourar{at}yahoo.com.

Chlamydia trachomatis is a common sexually transmitted pathogen. The impact of chlamydial infection on male infertility is controversial. The aim of this study was to assess the role of C. trachomatis human genital serovar E, on the sperm function, induction of apoptosis in spermatozoa and reproductive performance, using the Swiss male mice model. Fertile mice were inoculated in the meatus urethra with 106 C. trachomatis inclusion forming unit at day 0. The studied parameters were evaluated 7, 15, 21 and 30 days post inoculation (pi) in infected and sham-infected controls. Semen parameters of the infected mice groups were significantly lower than those of the control groups at the different days pi. DNA fragmentation study indicated that the mean percentages of apoptotic spermatozoa in the infected mice groups were significantly higher than those in the control groups 7 and 15 days pi, whereas the mean percentages of necrotic spermatozoa in the infected mice groups were significantly higher than those in the control group in the 30th day pi. A decrease of the reproductive performance was observed at different days pi in infected male mice groups when compared to the control groups. Furthermore, a statistically significant decrease in the mean number of infant mice was observed at 21 and 30 days pi. In conclusion, our data showed that inoculation of fertile male Swiss mice in the meatus urethra with C. trachomatis could lead to alteration of semen parameters, induction of apoptosis in spermatozoa and decrease of the reproductive performance of male mice.


Key words: Apoptosis • Chlamydia trachomatis • Reproductive performance • Semen parameters • Sexually transmitted disease and mice






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2010 by The American Society of Andrology.