Journal of Andrology
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Published-Ahead-of-Print February 4, 2010, DOI:10.2164/jandrol.109.008912

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Artay Yagci
William Murk
Jill Stronk
Gabor Huszar
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Spermatozoa Bound to Solid State Hyaluronic Acid Show Chromatin Structure with High DNA Chain Integrity: An Acridine Orange Fluorescence Study

Artay Yagci , William Murk , Jill Stronk , and Gabor Huszar *

* To whom correspondence should be addressed. E-mail: gabor.huszar{at}yale.edu.

During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step which facilitates formation of the zona pellucida and hyaluronic acid (HA)-binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona pellucida-bound sperm, including minimal DNA fragmentation, normal shape, and low frequency of chromosomal aneuploidies. In this work, we tested the hypothesis that HA-bound sperm, would be enhanced in sperm of high DNA chain integrity and green acridin orange fluorescence (AOF) as compared to the original sperm in semen. Sperm DNA integrity in semen and in their respective HA-bound sperm fractions was studied in 50 men tested for fertility workup. In the semen samples the proportions of sperm with green AOF (high DNA integrity) and red AOF (DNA breaks) were 54.9 ± 2.0% and 45.0 ± 1.9%, whereas in the HA-bound sperm fraction, the respective proportions were 99% and 1.0%. The data indeed demonstrated that HA show high degree of selectivity for sperm with high DNA integrity. These findings are important from the points of view of human sperm DNA integrity, sperm function, and the potential efficacy of hyaluronic acid mediated sperm selection for intracytoplasmic sperm injection.


Key words: Fertility • ICSI • IVF • Semen Analysis • Sperm • Acridin orange • DNA integrity • Sperm maturity • Sperm selection




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The effect of the swim-up and hyaluronan-binding methods on the frequency of abnormal spermatozoa detected by FISH and SCSA in carriers of balanced chromosomal translocations
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[Abstract] [Full Text] [PDF]


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