Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iwamoto, T.
Right arrow Articles by Gagnon, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iwamoto, T.
Right arrow Articles by Gagnon, C.

Journal of Andrology, Vol 9, Issue 6 377-383, Copyright © 1988 by The American Society of Andrology

JOURNAL ARTICLE

Purification and characterization of a sperm motility inhibitor in human seminal plasma

T. Iwamoto and C. Gagnon
Urology Research Laboratory, Royal Victoria Hospital, Montreal, Quebec, Canada.

A sperm motility inhibitor from human seminal plasma was purified and characterized. The purification procedure includes dialysis, ion exchange chromatography on SP-Sephadex C-25 and adsorption chromatography on hydroxylapatite. With this procedure, the seminal plasma motility inhibitor was purified 290-fold with a 24% recovery in inhibitory activity. Its molecular weight has been estimated at 18,000 to 22,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis, but at 13,000 to 15,000 according to molecular sieving under native conditions. The mobility inhibitor has an isoelectric point pH 9.1. It is stable over a wide range of pH (5 to 10) and at temperatures up to 60 C. The observation that the seminal plasma factor inhibited purified bull dynein ATPase in a concentration-dependent manner may suggest that it blocks the motility of demembranated spermatozoa by interfering with dynein arm function.


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
K. Yoshida, N. Kawano, M. Yoshiike, M. Yoshida, T. Iwamoto, and M. Morisawa
Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans
Mol. Hum. Reprod., March 1, 2008; 14(3): 151 - 156.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Kawano and M. Yoshida
Semen-Coagulating Protein, SVS2, in Mouse Seminal Plasma Controls Sperm Fertility
Biol Reprod, March 1, 2007; 76(3): 353 - 361.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Wang, E.E. Widgren, P. Sivashanmugam, M.G. O'Rand, and R.T. Richardson
Association of Eppin with Semenogelin on Human Spermatozoa
Biol Reprod, May 1, 2005; 72(5): 1064 - 1070.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Bergeron, M.-H. Crete, Y. Brindle, and P. Manjunath
Low-Density Lipoprotein Fraction from Hen's Egg Yolk Decreases the Binding of the Major Proteins of Bovine Seminal Plasma to Sperm and Prevents Lipid Efflux from the Sperm Membrane
Biol Reprod, March 1, 2004; 70(3): 708 - 717.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
K. Yoshida, T. Yamasaki, M. Yoshiike, S. Takano, I. Sato, and T. Iwamoto
Quantification of Seminal Plasma Motility Inhibitor/Semenogelin in Human Seminal Plasma
J Androl, November 1, 2003; 24(6): 878 - 884.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by The American Society of Andrology.