Journal of Andrology
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Journal of Andrology, Vol 8, Issue 5 349-353, Copyright © 1987 by The American Society of Andrology

JOURNAL ARTICLE

Correlation between defective motility (asthenospermia) and ATP reactivation of demembranated human spermatozoa

D. Y. Liu, M. G. Jennings and H. W. Baker
Medical Research Centre, Prince Henry's Hospital, Melbourne, Australia.

Human spermatozoa treated with 0.05% Triton X-100 to remove the cell membranes became immotile but flagellar movement was reinitiated by addition of 0.06 to 1 mM adenosine triphosphate (ATP). The percentage of spermatozoa showing flagellar movement 2 minutes after addition of 1 mM ATP from men with idiopathic asthenospermia (mean +/- SD, 34 +/- 15%), oligozoospermia (17 +/- 21%), sperm autoimmunity (17 +/- 12%), vasoepididymostomy (20 +/- 22%), or idiopathic zero motility (0%) was significantly lower than with spermatozoa from normal men (54 +/- 12%). The percentage of reactivated spermatozoa was correlated with the proportion of live cells (Eosin Y stain, r = 0.602, P less than 0.001), percentage of motile cells (r = 0.761, P less than 0.001), and motility index (r = 0.759, P less than 0.001) in the same semen samples. When expressed relative to initial sperm motility, the proportion reactivated was similar for idiopathic asthenospermia (85%) and normospermia (82%). Thus, failure to produce ATP does not appear to be a frequent cause of low sperm motility in man.




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Copyright © 1987 by The American Society of Andrology.