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Journal of Andrology, Vol 6, Issue 5 315-320, Copyright © 1985 by The American Society of Andrology

JOURNAL ARTICLE

Rat cauda epididymal fluid is a mucus

M. C. Usselman, R. A. Cone and D. P. Rossignol

Immobilin, the highly viscoelastic glycoprotein isolated from rat cauda epididymal fluid, exhibits all of the key biochemical characteristics of a mucin: 1) it has a very high molecular weight (will not pass through a 10(6) dalton cut-off filter; 2) it contains 56% carbohydrate, with low or undetectable levels of mannose, xylose and uronic acid; 3) the carbohydrates (primarily galactose, N-acetylglucosamine and N-acetylgalactosamine) are arranged in short, oligosaccharide chains (4-20 monosaccharides per chain); 4) these oligosaccharide chains can be cleaved by NaOH in the presence of NaBH4, suggesting O-glycosidic linkages; and 5) the protein core is pronase-resistant. Immobilin, however, contains no detectable sialic acid, and 67% of the oligosaccharides are uncharged, indicating that immobilin is less acidic than most other mucins.


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G. Frenette, C. Legare, F. Saez, and R. Sullivan
Macrophage migration inhibitory factor in the human epididymis and semen
Mol. Hum. Reprod., August 1, 2005; 11(8): 575 - 582.
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