Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Byers, S. W.
Right arrow Articles by Dym, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Byers, S. W.
Right arrow Articles by Dym, M.

Journal of Andrology, Vol 6, Issue 5 271-278, Copyright © 1985 by The American Society of Andrology

JOURNAL ARTICLE

Culture of ciliated and nonciliated cells from rat ductuli efferentes

S. W. Byers, N. A. Musto and M. Dym

The isolation and culture of ciliated and nonciliated cells from rat ductuli efferentes is described. Fragments of epithelium obtained after two collagenase digestions attached to plastic and to extracellular matrix and could be maintained in culture for at least 2 weeks. Ciliary beating in cells grown on epididymal extracellular matrix-coated plastic could be observed for up to 7 days in culture. Although cells maintained on this substrate retained organelles characteristic of cells in vivo, they assumed a flattened, squamous appearance. In contrast, cells growing on the surface of permeable supports impregnated with extracellular matrix were polarized and exhibited a cuboidal/columnar appearance. Androgen binding protein conjugated to colloidal gold was taken up by these cells via coated pits and was found sequentially in uncoated endosomes, multivesicular bodies and lysosomes.


This article has been cited by other articles:


Home page
 J AndrolHome page
Y. Araki, K. Suzuki, R. J. Matusik, M. Obinata, and M.-C. Orgebin-Crist
Immortalized Epididymal Cell Lines From Transgenic Mice Overexpressing Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene
J Androl, November 1, 2002; 23(6): 854 - 869.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by The American Society of Andrology.