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Journal of Andrology, Vol 3, Issue 6 434-439, Copyright © 1982 by The American Society of Andrology

Anti-Mouse Sperm Antiserum

Fertility Inhibition In Vitro and Preliminary Antigen Identification

PATRICIA M. SALING 1 AND MICHAEL G. O’RAND 1

1 Laboratories for Cell Biology, Department of Anatomy, University of North Carolina, Chapel Hill, North Carolina

Rabbit anti-mouse sperm (agrMS) Fab fragments have been prepared, which are capable of inhibiting spermzona interaction (both binding and penetration) and sperm-egg fusion in vitro. In cumulus-intact eggs, agrMS Fab did not inhibit zona penetration, but did inhibit sperm-egg fusion, implying that separate and distinguishable antigens are involved in these two fertilization events. Preliminary identification of the antigens recognized by aMS IgG, using a gel overlay technique, revealed four major antigens of 200 Kd, 55-63 Kd, 37 Kd, and 33 Kd, and three minor antigens of 110 Kd, 48 Kd, and 40 Kd.

     Key words: in vitro fertilization, anti-sperm antibodies

Accepted on June 3, 1982






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Copyright © 1982 by The American Society of Andrology.