Journal of Andrology
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Published-Ahead-of-Print April 4, 2007, DOI:10.2164/jandrol.106.001271
Journal of Andrology, Vol. 28, No. 5, September/October 2007
Copyright © American Society of Andrology
DOI: 10.2164/jandrol.106.001271

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Tag STS in the AZF Region Associated With Azoospermia in a Tunisian Population

LOBNA HADJKACEM-LOUKIL*, IMEN AYADI{dagger}, ALI BAHLOUL{ddagger}, HAMMADI AYADI* AND LEILA AMMAR-KESKES*

From the * Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax, Tunisia; {dagger} Centre of Biotechnology of Sfax, Tunisia; and {ddagger} Unit of Research US 17, CHU Hospital Habib Bourguiba, Sfax, Tunisia.

Correspondence to: Lobna Hadjkacem-Loukil, Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax, 3029, Tunisia (e-mail: lobna_h{at}lycos.com).


The aim of this study was to determine the appropriate tag sequence tagged site (STS) associated with azoospermia. We evaluated the incidence of Y chromosome microdeletions in Tunisian infertile male patients by polymerase chain reaction using 14 STSs in the azoospermia factor (AZF) region of Yq11. A logistic regression analysis was performed to test the association of STSs with semen quality. Haploview version 3.11 was used to identify the possible blocks of deletion involving a minimum number of STSs and that can be used to tag the deletion block in future analysis. Using the 14 STSs, 48% infertile patients (102 of 210) had microdeletions of Y chromosome but, following the European Academy of Andrology guidelines, only 16% of patients had microdeletions. A statistically significant difference was found with some STSs for azoospermia and oligozoospermia. A candidate combinaison composed of 4 STSs (RBMY-sy157-sy84-sy130) was associated with azoospermia in a Tunisian population. According to this study, this tag can be used in the screening of Y chromosome microdeletions before assisted reproduction treatment in a Tunisian population.

     Key words: Male infertility, Y chromosome microdeletions, Haploview






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Copyright © 2007 by The American Society of Andrology.