Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published-Ahead-of-Print August 23, 2006, DOI:10.2164/jandrol.106.000182
Journal of Andrology, Vol. 28, No. 1, January/February 2007
Copyright © American Society of Andrology
DOI: 10.2164/jandrol.106.000182

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
28/1/50    most recent
Author Manuscript (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jankowska, A.
Right arrow Articles by Sharma, R. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jankowska, A.
Right arrow Articles by Sharma, R. K.

Calcium-Modulated Rod Outer Segment Membrane Guanylate Cyclase Type 1 Transduction Machinery in the Testes

ANNA JANKOWSKA*, BEATA BURCZYNSKA*, TERESA DUDA{dagger}, JERZY B. WARCHOL* AND RAMESHWAR K. SHARMA{dagger}

From the * Department of Cell Biology, University of Medical Sciences, Poznan, Poland; and the {dagger} Unit of Regulatory and Molecular Biology, Departments of Cell Biology and Ophthalmology, SOM & NJMS, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey.

Correspondence to: Anna Jankowska, ul. Swiecickiego 6, 60-781 Poznan, Poland (e-mail: ajanko{at}amp.edu.pl).


The importance of the second messengers, Ca2+ and cyclic GMP, for the process of fertilization is well established; the mechanisms for their intracellular regulations in the testes are, however, poorly understood. This study documents the biochemical, molecular, and functional identity of a Ca2+-modulated membrane guanylate cyclase transduction machinery in bovine testes. The machinery is both inhibited and stimulated by free Ca2+ levels. The Ca2+-sensor component of the inhibitory mode of the machinery is GCAP1 (guanylate cyclase activating protein type 1) and for the stimulatory mode is S100B. The transduction component is a Ca2+-driven rod outer segment membrane guanylate cyclase type 1, ROS-GC1. The cyclase is predominantly expressed in spermatogenic cells. GCAP1 expression is restricted to a small population of spermatogonia, whereas S100B is present in the majority of spermatocytes and spermatids. The expression of GCAP1 and S100B in spermatocytes and spermatids is mutually exclusive.

     Key words: ROS-GC1, GCAP1, S100B, signal transduction






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by The American Society of Andrology.