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Journal of Andrology, Vol. 26, No. 4, July/August 2005
Copyright © American Society of Andrology
DOI: 10.2164/jandrol.04189

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Identification of Human HAPRIN Potentially Involved in the Acrosome Reaction

KOUICHI KITAMURA*, HIROMI NISHIMURA, YOSHITAKE NISHIMUNE AND HIROMITSU TANAKA

From the Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Disease, Osaka University, Osaka, Japan.
* Present address: Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.

Correspondence to: Dr Hiromitsu Tanaka, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan (e-mail: tanaka{at}biken.osaka-u.ac.jp).


The acrosome reaction in sperm is an exocytotic event required for fertilization. Previously, we isolated a novel haploid–germ-cell–specific gene in the mouse; this gene, named haprin, encodes the RING-finger, B-box–type zinc finger and coiled-coil domain (RBCC) motif protein and may be involved in the acrosome reaction. Here we report the molecular cloning and characterization of a human haprin ortholog. The deduced amino acid sequence of human haprin had 91% identity with the mouse ortholog. Transcripts of human haprin were detected exclusively in the testes. Western blot and immunocytochemical analyses detected HAPRIN protein in the testes and sperm. The protein was localized in the acrosomal region of sperm and disappeared after the acrosome reaction. Our results indicate that the function of HAPRIN is highly conserved in humans and mice and that the protein could play an important role in the regulation of the acrosome reaction.

     Key words: Sperm, spermatozoa, vesicle, RBCC protein






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