Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Christensen, P.
Right arrow Articles by Jensen, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Christensen, P.
Right arrow Articles by Jensen, J.
Journal of Andrology, Vol. 26, No. 1, January/February 2005
Copyright © American Society of Andrology

Relationship Between Sperm Viability as Determined by Flow Cytometry and Nonreturn Rate of Dairy Bulls

PREBEN CHRISTENSEN*, DOROTHEE BOELLING{dagger}, KURT MYRUP PEDERSEN*, INGE RIIS KORSGAARD{dagger} AND JUST JENSEN{dagger}

From the * Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Royal Veterinary and Agricultural University, Frederiksberg, Denmark; and the {dagger} Danish Institute of Animal Breeding and Genetics, Tjele, Denmark.

Correspondence to: Dr Preben Christensen, Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Royal Veterinary and Agricultural University, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark (e-mail: pc{at}kvl.dk).


A newly developed flow cytometric method for determination of sperm concentration and viability was tested in an insemination trial with cryopreserved bull sperm to establish the relationship between sperm viability and nonreturn rates. Semen for experimental inseminations was produced from 157 young sires (114 Holstein and 43 Jersey), each contributing 4 experimental semen collections. Straws containing approximately 15 x 106 motile sperm before freezing were used in 118 680 experimental inseminations performed by 254 artificial insemination technicians in 6352 Danish herds. Statistical analysis based on 44 946 experimental first inseminations showed that the major part (95.4%) of variation in the 56-day nonreturn rate (NRR56) was residual. Only 0.38% of the total variation in NRR56 was due to bulls and differences between ejaculate within bull. However, bulls were preselected, and a relatively high insemination dose was used. Correlations between sperm viability as assessed by flow cytometry and NRR56 was slightly lower than observed for microscopic assessment of sperm motility. However, flow cytometry makes it possible to achieve an objective and precise determination of sperm viability. It was therefore possible to calculate the effect on NRR56 provided selection of semen is based on the flow cytometric method. Three freezing extenders were used in this experiment, but a significant difference in NRR56 was not observed. Flow cytometric results for 1 extender (Biociphos Plus) indicated poorer sperm survival during postthaw incubation compared with Triladyl extender with whole and with clarified egg yolk.

     Key words: Spermatozoa, quality, insemination, fertility, precision, accuracy




This article has been cited by other articles:


Home page
 ReproductionHome page
A M Petrunkina, D Waberski, A R Gunzel-Apel, and E Topfer-Petersen
Determinants of sperm quality and fertility in domestic species
Reproduction, July 1, 2007; 134(1): 3 - 17.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by The American Society of Andrology.