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Identification of Differentially Expressed Genes in Mouse Spermatogenesis

YALI CHEN

YAN A. SU

XIAOQUAN LI

NEELAKANTA RAVINDRANATH

MARTIN DYM

WAI-YEE CHAN^*,,

From the ^* Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland; the Departments of Cell Biology and Pediatrics, Georgetown University, Washington, DC; and the Department of Pathology, Loyola University Medical Center, Maywood, Illinois.

Correspondence to: Dr Wai-Yee Chan, Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Dr, Room 2A08, MSC 4429, Bethesda, MD 20892-4429 (e-mail: chanwy{at}mail.nih.gov).

Complementary DNA microarray and quantitative polymerase chain reaction were used as tools for discovering genes that are differentially expressed in the mouse under normal physiological conditions at distinctive stages of male germ cell development, that is, type A spermatogonia, pachytene spermatocytes, and round spermatids. By using this strategy, we identified a set of genes exhibiting differential expression patterns in spermatogenesis, suggesting that specific functions of the encoded products occurred during the developmental process. Among them were several genes previously not known to be active in testis, which signified undiscovered functional roles of these genes during spermatogenesis. Many of the genes identified were not previously characterized. This study highlights new targets for manipulation to unravel the molecular mechanism of spermatogenesis.

     Key words: Type A spermatogonia, pachytene spermatocytes, round spermatids, spermatogenesis, complementary DNA microarray, quantitative polymerase chain reaction

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