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From the * Department of Veterinary Biosciences,
University of Illinois, Urbana, Illinois;
Department of Urology, College of Medicine,
University of Illinois, Chicago, Illinois; MRC
Human Reproductive Sciences Unit, Edinburgh EH3 9ET, United Kingdom;
Departments of Cell and Structural Biology, and
Molecular and Integrative Physiology, University of Illinois, Urbana,
Illinois.
| Correspondence to: Dr Rex A. Hess, Veterinary Biosciences, University of Illinois, 2001 S. Lincoln, Urbana, IL 61802-6199 (e-mail: r-hess{at}uiuc.edu). |
and ERß were visualized by immunohistochemistry in adult male mice
reproductive tracts, including testes, efferent ductules; initial segment,
caput, corpus, and cauda epididymides; and vas deferens. Antibody specificity
was demonstrated by Western blot and antibody competition. In testis, AR was
expressed in Leydig cells, Sertoli cells, and most peritubular cells, but not
in germ cells; Sertoli cells showed more intense staining in stages VI-VII;
ER was present in Leydig and some peritubular cells; ERß was in
Leydig, some peritubular, all Sertoli and germ cells except in spermatids and
meiotic spermatocytes. In efferent ductules, AR was strongly expressed in
ciliated and nonciliated epithelial cells and in stromal cells; ER was
strongly expressed in ciliated and nonciliated epithelial cells; stromal cells
were negative; and ERß was strongly expressed in ciliated and nonciliated
epithelial cells and also in stromal cells. In epididymis, AR was strongly
expressed in all epithelial cells (not in intraepithelial lymphocytes);
ER was strongly expressed in apical, narrow, and some basal cells of
the initial segment, and in caput, principal cells of the caput, clear cells
of the distal caput through cauda; stromal cells were negative in the initial
segment, but more stromal cells were stained from caput to cauda; ERß was
strongly expressed in most of epithelial cells of the epididymis, but stromal
cells were inconsistently stained. In vas deferens, AR was weakly expressed or
absent in principal cells but moderately stained in basal cells, smooth muscle
cells of stroma were stained intensely, ER was absent in epithelial
cells but present in a subepithelial smooth muscle layer, and ERß was
strongly expressed in all epithelial cells and most stromal cells. This study
demonstrates that the reproductive tracts of male mice differ considerably
from those of rats in expression of ARs and ERs and that caution is needed
when extrapolating nuclear steroid receptor data across mammalian species.
Key words: Efferent ductule, epididymis, steroid hormone receptors, testis, vas deferens
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