Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mueller, S. O.
Right arrow Articles by Korach, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mueller, S. O.
Right arrow Articles by Korach, K. S.

Journal of Andrology, Vol 22, Issue 4 652-664, Copyright © 2001 by The American Society of Andrology

JOURNAL ARTICLE

Immortalized testis cell lines from estrogen receptor (ER) alpha knock-out and wild-type mice expressing functional ERalpha or ERbeta

S. O. Mueller and K. S. Korach
Laboratory of Reproductive and Developmental Toxicology-Receptor Biology Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA. stefan-otto.mueller@knoll-ag.de

The surprising findings that male mice lacking the estrogen receptor (ER) alpha (alphaERKO) have atrophic testis and are infertile proved that ERalpha is involved in normal testicular function. To obtain compatible in vitro model systems for alphaERKO male mice, we immortalized different cell lines from the testis of alphaERKO and wild-type (C57BL/6) mice with the human papilloma virus E6/E7 genes. The established cell lines were characterized for Sertoli, Leydig, and peritubular cell markers by means of messenger RNA expression and functional assays. One wild-type-derived cell line showed Leydig cell-specific marker gene expression and produced testosterone after stimulation with cyclic adenosine monophosphate. Most wild-type cell lines expressed androgen receptor and a functional ERalpha as shown by high estrogenic activity in a luciferase-based transactivation assay. Most notably, the wild-type-derived WL3, and the ES4 cell line derived from alphaERKO mice expressed ERbeta and showed ER-mediated transcriptional activity, but no ERalpha protein expression. These cell lines with and without functional ERalpha or ERbeta enable the analyses of ER subtype-specific responses and their function in testicular cell signaling, morphogenesis, and neoplasia.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. O. Mueller, J. M. Hall, D. L. Swope, L. C. Pedersen, and K. S. Korach
Molecular Determinants of the Stereoselectivity of Agonist Activity of Estrogen Receptors (ER) alpha and beta
J. Biol. Chem., March 28, 2003; 278(14): 12255 - 12262.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. O. Mueller, J. A. Clark, P. H. Myers, and K. S. Korach
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor {alpha}
Endocrinology, June 1, 2002; 143(6): 2357 - 2365.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by The American Society of Andrology.