Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Way, A. L.
Right arrow Articles by Killian, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Way, A. L.
Right arrow Articles by Killian, G. J.

Journal of Andrology, Vol 21, Issue 2 213-219, Copyright © 2000 by The American Society of Andrology

JOURNAL ARTICLE

Effects of accessory sex gland fluid on viability, capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa

A. L. Way, L. C. Griel Jr and G. J. Killian
Department of Dairy and Animal Science, J.O. Almquist Research Center, Pennsylvania State University, University Park 16802, USA.

The effect of accessory sex gland fluid (AGF) on viability and acrosomal integrity of spermatozoa was examined with cauda epididymal spermatozoa and AGF from the same Holstein bull (n = 6). Surgical cannulation of the vasa deferentia enabled the separate collection of cauda epididymal effluent and AGF from each bull. Cauda epididymal effluent was incubated with either AGF collected from the same bull or medium alone. Following coincubation, spermatozoa (5 x 10(7) sperm/mL) were incubated in medium alone or under capacitating conditions (10 microg/mL heparin) for 16 hours. Every 2 hours, an aliquot of spermatozoa was exposed to lysophosphatidylcholine (100 microg/mL) to induce the acrosome reaction in capacitated spermatozoa. Sperm motility decreased over time regardless of treatment. Overall, spermatozoa incubated in AGF had fewer acrosome-intact live spermatozoa than did those not incubated in AGF. Viability was significantly (P < .05) compromised over time when spermatozoa were exposed to AGF, compared with those not preincubated in AGF. Significantly more (P < .05) acrosome-reacted live spermatozoa were seen following exposure to heparin and lysophosphatidylcholine when spermatozoa were not preincubated in AGF. We conclude that exposure of spermatozoa to AGF accelerates cell death and that rapid removal of spermatozoa from seminal plasma is critical for maximal viability.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
A. Bergeron, M.-H. Crete, Y. Brindle, and P. Manjunath
Low-Density Lipoprotein Fraction from Hen's Egg Yolk Decreases the Binding of the Major Proteins of Bovine Seminal Plasma to Sperm and Prevents Lipid Efflux from the Sperm Membrane
Biol Reprod, March 1, 2004; 70(3): 708 - 717.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. Manjunath, V. Nauc, A. Bergeron, and M. Menard
Major Proteins of Bovine Seminal Plasma Bind to the Low-Density Lipoprotein Fraction of Hen's Egg Yolk
Biol Reprod, October 1, 2002; 67(4): 1250 - 1258.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
A. L. Way and G. J. Killian
Capacitation and Induction of the Acrosome Reaction in Bull Spermatozoa With Norepinephrine
J Androl, May 1, 2002; 23(3): 352 - 357.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by The American Society of Andrology.