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1 School of Medicine and
Department of Physiology, Southern
Illinois University, Carbondale, Illinois
Two methods were used to assess the ability of
plasma membrane (PM) polypeptides to bind to
isolated zonae pellucidae (ZP). Crossed immunoelectrophoresis patterns of detergent
solubilized PM were compared in the presence
and absence of ZP. Two antigens which represented less than 20% of the total antigen response were significantly changed in electrophoretic mobility and precipitin peak height.
In the second experiment, detergent solubilized PMs, labeled with iodine-125 (I125), were
absorbed to a column containing purified ZP.
Washed columns eluted with SDS-urea were
found to be enriched in several polypeptides
which migrate in PAGE-SDS gels below 22 Kd.
Comparison of PAGE patterns of PM originating
primarily from the sperm head with those enriched in PM from other regions of the sperm
surface suggest that these low molecular
weight polypeptides with affinity for isolated ZP
are concentrated in the sperm head. These experiments provide a base for determining
which PM components are involved in spermegg interactions in vivo.
Key words: sperm, plasma membrane, zonae pellucidae
Submitted on February 17, 1981
Revised on May 8, 1981
Accepted on June 2, 1981
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