| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Andrology, Vol 19, Issue 4 385-393, Copyright © 1998 by The American Society of Andrology
JOURNAL ARTICLE |
J. E. Welch, R. R. Barbee, N. L. Roberts, J. D. Suarez and G. R. Klinefelter
United States Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Reproductive Toxicology Division, Research Triangle Park, North Carolina 27711, USA.
Three nucleotide sequences encoding SP22, a protein originally identified in detergent extracts of cauda epididymal sperm, were isolated from a rat testis cDNA library. While two of these cDNA sequences differed only in their 5' untranslated regions, a third cDNA was predicted to contain an additional 13 amino acids of coding sequence. Amino acid sequences obtained following Edman degradation of purified SP22 protein and cDNA sequence data both indicated that SP22 was a member of a highly conserved and widely expressed gene family found in organisms as diverse as human and Escherichia coli. Interestingly, while a 1-kb mRNA transcript was widely expressed in somatic tissues, a unique pattern of testicular expression was observed, including the appearance of a novel 1.5-kb transcript and an increase in the abundance of the 1-kb transcript during spermatogenic cell development. Anti-SP22 peptide antiserum was shown to recognize a family of 22-kDa proteins on western blots of detergent-extracted cauda epididymal sperm protein, suggesting that multiple charge variants of SP22 coexist. Moreover, affinity-purified anti-SP22 peptide immunoglobulin localized in a highly specific manner to the anterior-ventral surface of the equatorial segment of the sperm head. This is an extremely intriguing finding as SP22 was originally shown to be highly correlated with, and predictive of, the fertilizing ability of cauda epididymal sperm. Although no conclusive function has been attributed to any members of the SP22 gene family, the localization of SP22 over a discrete region of the sperm head suggests a pivotal role in sperm-egg interactions.
This article has been cited by other articles:
 |
|
 |
|
  A. M. Benoit, H. A. LaVoie, G. L. McCoy, and C. A. Blake Expression of Sperm Protein 22 (SP22) in the Rat Ovary During Different Reproductive States Experimental Biology and Medicine, July 1, 2007; 232(7): 910 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. R. Veeramachaneni, J. S. Palmer, and G. R. Klinefelter Chronic Exposure to Low Levels of Dibromoacetic Acid, a Water Disinfection By-product, Adversely Affects Reproductive Function in Male Rabbits J Androl, July 1, 2007; 28(4): 565 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Lucas and I. Marin A New Evolutionary Paradigm for the Parkinson Disease Gene DJ-1 Mol. Biol. Evol., February 1, 2007; 24(2): 551 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Benoit, G. L. McCoy, and C. A. Blake Localization of Fertility Factor SP22 to Specific Cell Types Within the Anterior Pituitary Gland Experimental Biology and Medicine, November 1, 2005; 230(10): 721 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ooe, T. Taira, S. M. M. Iguchi-Ariga, and H. Ariga Induction of Reactive Oxygen Species by Bisphenol A and Abrogation of Bisphenol A-Induced Cell Injury by DJ-1 Toxicol. Sci., November 1, 2005; 88(1): 114 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Kaydos, J. D. Suarez, N. L. Roberts, K. Bobseine, R. Zucker, J. Laskey, and G. R. Klinefelter Haloacid Induced Alterations in Fertility and the Sperm Biomarker SP22 in the Rat Are Additive: Validation of an ELISA Toxicol. Sci., October 1, 2004; 81(2): 430 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Olzmann, K. Brown, K. D. Wilkinson, H. D. Rees, Q. Huai, H. Ke, A. I. Levey, L. Li, and L.-S. Chin Familial Parkinson's Disease-associated L166P Mutation Disrupts DJ-1 Protein Folding and Function J. Biol. Chem., February 27, 2004; 279(9): 8506 - 8515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Tao and L. Tong Crystal Structure of Human DJ-1, a Protein Associated with Early Onset Parkinson's Disease J. Biol. Chem., August 15, 2003; 278(33): 31372 - 31379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Honbou, N. N. Suzuki, M. Horiuchi, T. Niki, T. Taira, H. Ariga, and F. Inagaki The Crystal Structure of DJ-1, a Protein Related to Male Fertility and Parkinson's Disease J. Biol. Chem., August 15, 2003; 278(33): 31380 - 31384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bohring and W. Krause Immune infertility: towards a better understanding of sperm (auto)-immunity: The value of proteomic analysis Hum. Reprod., May 1, 2003; 18(5): 915 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Klinefelter, L. F. Strader, J. D. Suarez, and N. L. Roberts Bromochloroacetic Acid Exerts Qualitative Effects on Rat Sperm: Implications for a Novel Biomarker Toxicol. Sci., July 1, 2002; 68(1): 164 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takahashi, T. Taira, T. Niki, C. Seino, S. M. M. Iguchi-Ariga, and H. Ariga DJ-1 Positively Regulates the Androgen Receptor by Impairing the Binding of PIASxalpha to the Receptor J. Biol. Chem., September 28, 2001; 276(40): 37556 - 37563. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
