Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jiang, C.
Right arrow Articles by Boekelheide, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jiang, C.
Right arrow Articles by Boekelheide, K.

Journal of Andrology, Vol 18, Issue 4 393-399, Copyright © 1997 by The American Society of Andrology

JOURNAL ARTICLE

Development and characterization of a prepubertal rat Sertoli cell line, 93RS2

C. Jiang, S. J. Hall and K. Boekelheide
Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island 02912, USA.

Sertoli cells in the seminiferous epithelium provide both structural and nutritional support to germ cells during spermatogenesis. Primary Sertoli cells in culture are an effective tool for the in vitro study of Sertoli cell function; however, primary cultures are inherently variable, time consuming to prepare, expensive, and wasteful of animals. We therefore developed a Sertoli cell line, called 93RS2, by immortalizing primary Sertoli cells derived from prepubertal rats with SV40 tsA255. This cell line proliferates at the permissive temperature (32 degrees C) and has enhanced expression of a differentiated Sertoli cell phenotype at the nonpermissive temperature (40-41 degrees C). Cytogenetic analysis demonstrated that 93RS2 has 42 chromosomes per cell, the same as a normal rat. mRNA analysis showed that this cell line, when cultured at a nonpermissive temperature, exhibited increased expression of transferrin in the presence of testosterone and enhanced expression of sulfated glycoprotein-2. A tumorigenicity assay showed that 93RS2 cells were temperature-dependent for growth in soft agar and were capable of forming tumors in nude mice. In conclusion, this rat 93RS2 cell line should be useful for the study of Sertoli cell function.


This article has been cited by other articles:


Home page
 J AndrolHome page
S. A. Beall, K. Boekelheide, and K. J. Johnson
Hybrid GPCR/Cadherin (Celsr) Proteins in Rat Testis Are Expressed With Cell Type Specificity and Exhibit Differential Sertoli Cell-Germ Cell Adhesion Activity
J Androl, July 1, 2005; 26(4): 529 - 538.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
M.-C. Hofmann, K. S. Van Der Wee, J. L. Dargart, G. Dirami, L. Dettin, and M. Dym
Establishment and Characterization of Neonatal Mouse Sertoli Cell Lines
J Androl, January 1, 2003; 24(1): 120 - 130.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by The American Society of Andrology.