| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Andrology, Vol 17, Issue 4 394-402, Copyright © 1996 by The American Society of Andrology
JOURNAL ARTICLE |
K. Henriksen, J. Kulmala, J. Toppari, K. Mehrotra and M. Parvinen
Department of Anatomy, University of Turku, Finland.
The effects of 3 Gy local X-irradiation on the adult rat testis were studied together with exact determination of the radiation dose distribution in the testis. Seminiferous tubule segments were isolated 8-66 hours postirradiation (p.i.), squashed between a microscope slide and a coverslip, and the exact stage of the seminiferous epithelial cycle was identified under a phase-contrast microscope. The squash preparations were subjected to in situ end labeling (ISEL) for visualization and quantification of apoptotic cells. In controls, the highest numbers of apoptotic cells were scored in stages XII-XIV and I. In situ end-label staining of cells was observed in A3-A4 spermatogonia, spermatocytes at zygotene, pachytene, and meiotic division phases, as well as in early spermatids. In irradiated testes, from 8 hours p.i. and onward, intermediate- and B-type spermatogonia were sensitive at stages II-VI. At 42 hours, in stage I, elevated numbers of degenerating spermatocytes were seen. Most of them had not undergone meiotic divisions at stage XIV and showed an apoptotic type of degeneration at stage I. At the time of irradiation, the cells were in stage XIII, suggesting that diakinetic spermatocytes are particularly sensitive to irradiation. Also, preleptotene-zygotene spermatocytes in stages VII-XII were sensitive to irradiation. Apoptotic-type of cell degeneration was confirmed by living cell squash preparations, electron microscopy, and DNA electrophoresis. In conclusion, irradiation may provide a useful model system for studying apoptosis, and its control in spermatogonia and meiotically dividing cells.
This article has been cited by other articles:
 |
|
 |
|
  N. E. Furland, S. R. Zanetti, G. M. Oresti, E. N. Maldonado, and M. I. Aveldano Ceramides and Sphingomyelins with High Proportions of Very Long-chain Polyunsaturated Fatty acids in Mammalian Germ Cells J. Biol. Chem., June 22, 2007; 282(25): 18141 - 18150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J Meachem, S. M Ruwanpura, J. Ziolkowski, J. M Ague, M. K Skinner, and K. L Loveland Developmentally distinct in vivo effects of FSH on proliferation and apoptosis during testis maturation J. Endocrinol., September 1, 2005; 186(3): 429 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Seli and D. Sakkas Spermatozoal nuclear determinants of reproductive outcome: implications for ART Hum. Reprod. Update, July 1, 2005; 11(4): 337 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. V. Andreu-Vieyra, A. G. Buret, and H. R. Habibi Gonadotropin-Releasing Hormone Induction of Apoptosis in the Testes of Goldfish (Carassius auratus) Endocrinology, March 1, 2005; 146(3): 1588 - 1596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Otala, M. O. Pentikainen, T. Matikainen, L. Suomalainen, J. K. Hakala, G. I. Perez, M. Tenhunen, K. Erkkila, P. Kovanen, M. Parvinen, et al. Effects of Acid Sphingomyelinase Deficiency on Male Germ Cell Development and Programmed Cell Death Biol Reprod, January 1, 2005; 72(1): 86 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Otala, L. Suomalainen, M. O. Pentikainen, P. Kovanen, M. Tenhunen, K. Erkkila, J. Toppari, and L. Dunkel Protection from Radiation-Induced Male Germ Cell Loss by Sphingosine-1-Phosphate Biol Reprod, March 1, 2004; 70(3): 759 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Francavilla, P. D'Abrizio, G. Cordeschi, F. Pelliccione, S. Necozione, S. Ulisse, G. Properzi, and F. Francavilla Fas expression correlates with human germ cell degeneration in meiotic and post-meiotic arrest of spermatogenesis Mol. Hum. Reprod., March 1, 2002; 8(3): 213 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jansen, A. K. Olsen, R. Wiger, H. Naegeli, P. de Boer, F. van der Hoeven, J. A. Holme, G. Brunborg, and L. Mullenders Nucleotide excision repair in rat male germ cells: low level of repair in intact cells contrasts with high dual incision activity in vitro Nucleic Acids Res., April 15, 2001; 29(8): 1791 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Francavilla, P. D’Abrizio, N. Rucci, G. Silvano, G. Properzi, E. Straface, G. Cordeschi, S. Necozione, L. Gnessi, M. Arizzi, et al. Fas and Fas Ligand Expression in Fetal and Adult Human Testis with Normal or Deranged Spermatogenesis J. Clin. Endocrinol. Metab., August 1, 2000; 85(8): 2692 - 2700. [Abstract] [Full Text]
|
|
|
|
|
|
K. Jahnukainen, M. Hou, M. Parvinen, S. Eksborg, and O. Söder Stage-Specific Inhibition of Deoxyribonucleic Acid Synthesis and Induction of Apoptosis by Antracyclines in Cultured Rat Spermatogenic Cells Biol Reprod, August 1, 2000; 63(2): 482 - 487. [Abstract] [Full Text]
|
|
|
|
 |
|
 W Yan, J Suominen, and J Toppari Stem cell factor protects germ cells from apoptosis in vitro J. Cell Sci., January 1, 2000; 113(1): 161 - 168. [Abstract] [PDF]
|
|
|
|
 |
|
 V. Pentikainen, K. Erkkila, and L. Dunkel Fas regulates germ cell apoptosis in the human testis in vitro Am J Physiol Endocrinol Metab, February 1, 1999; 276(2): E310 - E316. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
