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Journal of Andrology, Vol 17, Issue 2 151-157, Copyright © 1996 by The American Society of Andrology

JOURNAL ARTICLE

Effect of sperm lipid peroxidation on fertilization

H. Kodama, Y. Kuribayashi and C. Gagnon
Urology Research Laboratory, Royal Victoria Hospital, Montreal, Canada.

This study was aimed at determining, with homologous mouse gametes, whether a level of membrane lipid peroxidation insufficient to affect sperm motility parameters can alter sperm fertilizing potential. The addition of ferrous ions and ascorbic acid (Fe2+/ Asc) to mouse sperm suspensions increases the formation of thiobarbituric acid-reactive substances (TBARS), an indicator of lipid peroxide breakdown products, without significantly affecting the level of sulfhydryl groups. In the presence of Fe2+/Asc concentrations above > 0.4/2.0 mM, spermatozoa become immotile. However, at concentrations < or = 0.4/2.0 mM of Fe2+/Asc, i.e., conditions in which the TBARS formation is increased by < or = 4.6-fold over that of controls, motility remains unaffected for up to 3 hours. In the presence of 0.4/ 2.0 mM Fe2+/Asc, treated spermatozoa increase their fertilizing potential by 50%, as measured by the formation of two-cell embryos. This increase is not caused by improvements in sperm motility parameters (percentage, linearity, velocity, hyperactivation) or sperm capacitation. On the other hand, there is a significant increase in the capacity of mouse spermatozoa to bind to homologous zona pellucida. In conclusion, mild peroxidative conditions, that increase lipid peroxide formation 4.6-fold without significantly modifying free sulfhydryl groups and sperm motility parameters, improve the fertilizing potential of spermatozoa by increasing their binding capacity to zona pellucida.


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Copyright © 1996 by The American Society of Andrology.