Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roth, T. L.
Right arrow Articles by Wildt, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roth, T. L.
Right arrow Articles by Wildt, D. E.

Journal of Andrology, Vol 15, Issue 2 165-173, Copyright © 1994 by The American Society of Andrology

JOURNAL ARTICLE

Zona pellucida piercing enhances zona penetration by spermatozoa from normospermic and teratospermic domestic cats

T. L. Roth, J. Howard and D. E. Wildt
National Zoological Park, Smithsonian Institution, Washington, DC 20008.

Poor quality ejaculates appear associated with reduced zona penetration and poor fertilization in vitro in some felid species or populations. Even morphologically normal sperm from these teratospermic ejaculates are compromised in ability to penetrate the zona pellucida and fertilize oocytes. A domestic cat model and zona piercing were used to study the function of normal and malformed sperm. Male cats naturally producing different proportions of morphologically normal sperm (group I, > 60%; group II, 45-55%; group III, < 40%) were identified. Electroejaculates were subjected to sperm washing (WS) and half of these to swim-up (SU) processing following WS, and all samples were assessed for a sperm motility index (SMI) over time. Zonae of salt-stored (SS), domestic cat oocytes were mechanically pierced (ZnPd) three times each. Control (nonmanipulated) SS oocytes (n = 430) and ZnPd-SS oocytes (n = 430) were coincubated with sperm in vitro. The proportion of morphologically normal sperm/ejaculate differed (P < 0.05) among groups averaging 68.8% for group I, 53.5% for group II, and 34.7% for group III. After SU processing, the percent normal sperm among groups was similar (P > 0.05; range, 70.8-80.2%). Average SMI for all groups was high (> 70) at 0 hour and decreased over time, but the descent of slope was greater (P < 0.05) for group III than groups I and II. The proportion of oocytes penetrated was not influenced (P > 0.05) by sperm-processing treatment. For all groups, inner zona penetration was greater (P < 0.05) in ZnPd-SS than SS oocytes (group I, 52.3% vs. 34.9%; group II, 37.9% vs. 20.3%; group III, 32.0% vs. 17.2%).(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
K. Neubauer, K. Jewgenow, S. Blottner, D. E. Wildt, and B. S. Pukazhenthi
Quantity Rather Than Quality in Teratospermic Males: A Histomorphometric and Flow Cytometric Evaluation of Spermatogenesis in the Domestic Cat (Felis catus)
Biol Reprod, November 1, 2004; 71(5): 1517 - 1524.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. M. Penfold, L. Jost, D. P. Evenson, and D. E. Wildt
Normospermic Versus Teratospermic Domestic Cat Sperm Chromatin Integrity Evaluated by Flow Cytometry and Intracytoplasmic Sperm Injection
Biol Reprod, November 1, 2003; 69(5): 1730 - 1735.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. E. Spindler and D. E. Wildt
Circannual Variations in Intraovarian Oocyte but Not Epididymal Sperm Quality in the Domestic Cat
Biol Reprod, July 1, 1999; 61(1): 188 - 194.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by The American Society of Andrology.