| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Andrology, Vol 14, Issue 2 87-98, Copyright © 1993 by The American Society of Andrology
JOURNAL ARTICLE |
C. Pineau, V. Syed, C. W. Bardin, B. Jegou and C. Y. Cheng
Population Council, New York, New York 10021.
In view of the evidence showing that germ cells regulate Sertoli cell (SC) function, the aim of this study was to examine if germ cell (GC)-conditioned media contained multiple biological factors that affect SC secretory functions. Total GC were isolated from adult rat testes. Pachytene spermatocytes (SPC) and early spermatids (SPT) were enriched to about 90% pure by centrifugal elutriation. GC, SPC, and SPT were cultured in serum-free medium for 20 hours with a viability greater than 95%. Conditioned media derived from these cells were fractionated by anion-exchange high-performance liquid chromatography (HPLC). An aliquot from each of these fractions was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were visualized by silver staining. The patterns of protein staining using media from GC, SPC, and SPT were similar. Bioassays of these column fractions on SC showed that the transferrin stimulatory, the testins inhibitory, and the clusterin inhibitory activities were eluted from the anion-exchange HPLC column in overlapping fractions. To determine whether these activities were confined to one or several molecules, further fractionations were performed. Eleven liters of GC-conditioned medium were fractionated by sequential HPLC using anion-exchange, gel permeation, and reversed-phase columns. Throughout the entire fractionation scheme, the HPLC fractions were bioassayed using primary SC-enriched cultures prepared from 20-day-old rats by incubating SC with aliquots of these fractions for 24 hours to monitor their effects on SC secretory function. The concentrations of transferrin, clusterin, and testins were quantified by specific radioimmunoassays. These studies showed that the transferrin stimulatory activity can be fractionated into four peaks (I, IIa, IIb, and IIc); clusterin inhibitory activity into three peaks (A, B, and C); and testins inhibitory activity into two peaks (1 and 2). Some of these bioactivities were eluted in overlapping fractions such as I and B, IIb and 1, and IIc and 2, whereas A, C, and IIa were not associated with any other assayed activities. In summary, additional GC modulators of SC function were identified for the first time, these include clusterin and testins inhibitors. The previously identified transferrin stimulatory activity was also resolved into multiple molecular forms.
This article has been cited by other articles:
 |
|
 |
|
  P. P. Y. Lie, D. D. Mruk, W. M. Lee, and C. Y. Cheng Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium FASEB J, August 1, 2009; 23(8): 2555 - 2567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J.K. Anand-Ivell, V. Relan, M. Balvers, I. Coiffec-Dorval, M. Fritsch, R. A.D. Bathgate, and R. Ivell Expression of the Insulin-Like Peptide 3 (INSL3) Hormone-Receptor (LGR8) System in the Testis Biol Reprod, May 1, 2006; 74(5): 945 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Dorval-Coiffec, J.-G. Delcros, H. Hakovirta, J. Toppari, B. Jegou, and C. Piquet-Pellorce Identification of the Leukemia Inhibitory Factor Cell Targets Within the Rat Testis Biol Reprod, March 1, 2005; 72(3): 602 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Guais, B. Solhonne, N. Melaine, G. Guellaen, and F. Bulle Goliath, a Ring-H2 Mitochondrial Protein, Regulated by Luteinizing Hormone/Human Chorionic Gonadotropin in Rat Leydig Cells Biol Reprod, January 1, 2004; 70(1): 204 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Habasque, A.-P. Satie, F. Aubry, B. Jegou, and M. Samson Expression of fractalkine in the rat testis: molecular cloning of a novel alternative transcript of its gene that is differentially regulated by pro-inflammatory cytokines Mol. Hum. Reprod., August 1, 2003; 9(8): 449 - 455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-y. Lui, W. M. Lee, and C. Y. Cheng Sertoli-Germ Cell Adherens Junction Dynamics in the Testis Are Regulated by RhoB GTPase via the ROCK/LIMK Signaling Pathway Biol Reprod, June 1, 2003; 68(6): 2189 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Y. Siu, D. D. Mruk, W. M. Lee, and C. Y. Cheng Adhering Junction Dynamics in the Testis Are Regulated by an Interplay of {beta}1-Integrin and Focal Adhesion Complex-Associated Proteins Endocrinology, May 1, 2003; 144(5): 2141 - 2163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. N. Lau and D. D. Mruk Rab8B GTPase and Junction Dynamics in the Testis Endocrinology, April 1, 2003; 144(4): 1549 - 1563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oyhenart, R. Le Goffic, M. Samson, B. Jegou, and N. Raich Phtf1 Is an Integral Membrane Protein Localized in an Endoplasmic Reticulum Domain in Maturing Male Germ Cells Biol Reprod, March 1, 2003; 68(3): 1044 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P.Y. Lee, D. Mruk, W. M. Lee, and C. Y. Cheng Is the Cadherin/Catenin Complex a Functional Unit of Cell-Cell Actin-Based Adherens Junctions in the Rat Testis? Biol Reprod, February 1, 2003; 68(2): 489 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Y. Siu, W. M. Lee, and C. Y. Cheng The Interplay of Collagen IV, Tumor Necrosis Factor-{alpha}, Gelatinase B (Matrix Metalloprotease-9), and Tissue Inhibitor of Metalloproteases-1 in the Basal Lamina Regulates Sertoli Cell-Tight Junction Dynamics in the Rat Testis Endocrinology, January 1, 2003; 144(1): 371 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Com, F. Bourgeon, B. Evrard, T. Ganz, D. Colleu, B. Jegou, and C. Pineau Expression of Antimicrobial Defensins in the Male Reproductive Tract of Rats, Mice, and Humans Biol Reprod, January 1, 2003; 68(1): 95 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Melaine, M.-O. Lienard, I. Dorval, C. Le Goascogne, H. Lejeune, and B. Jegou Multidrug Resistance Genes and P-Glycoprotein in the Testis of the Rat, Mouse, Guinea Pig, and Human Biol Reprod, December 1, 2002; 67(6): 1699 - 1707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.D. Gong, J.C.H. Li, G.P.H. Leung, K.H. Cheung, and P.Y.D. Wong A BKCa to Kv Switch During Spermatogenesis in the Rat Seminiferous Tubules Biol Reprod, July 1, 2002; 67(1): 46 - 54. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Habasque, F. Aubry, B. Jegou, and M. Samson Study of the HIV-1 receptors CD4, CXCR4, CCR5 and CCR3 in the human and rat testis Mol. Hum. Reprod., May 1, 2002; 8(5): 419 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yan, M. Samson, B. Jégou, and J. Toppari Bcl-w Forms Complexes with Bax and Bak, and Elevated Ratios of Bax/Bcl-w and Bak/Bcl-w Correspond to Spermatogonial and Spermatocyte Apoptosis in the Testis Mol. Endocrinol., May 1, 2000; 14(5): 682 - 699. [Abstract] [Full Text]
|
|
|
|
|
|
F. Aubry, C. Habasque, A.-P. Satie, B. Jégou, and M. Samson Expression and Regulation of the CC-Chemokine Monocyte Chemoattractant Protein-1 in Rat Testicular Cells in Primary Culture Biol Reprod, May 1, 2000; 62(5): 1427 - 1435. [Abstract] [Full Text]
|
|
|
|
|
|
C. Piquet-Pellorce, I. Dorval-Coiffec, M.-D. Pham, and B. Jegou Leukemia Inhibitory Factor Expression and Regulation within the Testis Endocrinology, March 1, 2000; 141(3): 1136 - 1141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. Samy, J. C. H. Li, J. Grima, W. M. Lee, B. Silvestrini, and C. Y. Cheng Sertoli Cell Prostaglandin D2 Synthetase Is a Multifunctional Molecule: Its Expression and Regulation Endocrinology, February 1, 2000; 141(2): 710 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Pineau, S McCool, M. Glucksman, B Jegou, and A. Pierotti Distribution of thimet oligopeptidase (E.C. 3.4.24.15) in human and rat testes J. Cell Sci., January 10, 1999; 112(20): 3455 - 3462. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Grima, C. C. S. Wong, L.-j. Zhu, S.-d. Zong, and C. Y. Cheng Testin Secreted by Sertoli Cells Is Associated with the Cell Surface, and Its Expression Correlates with the Disruption of Sertoli-Germ Cell Junctions but Not the Inter-Sertoli Tight Junction J. Biol. Chem., August 14, 1998; 273(33): 21040 - 21053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dejucq, S. Chousterman, and B. Jegou The Testicular Antiviral Defense System: Localization, Expression, and Regulation of 2'5' Oligoadenylate Synthetase, Double-Stranded RNA-activated Protein Kinase, and Mx Proteins in the Rat Seminiferous Tubule J. Cell Biol., November 17, 1997; 139(4): 865 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Aravindan, D. Mruk, W. M. Lee, and C. Y. Cheng Identification, Isolation, and Characterization of a 41-Kilodalton Protein from Rat Germ Cell-Conditioned Medium Exhibiting Concentration-Dependent Dual Biological Activities Endocrinology, August 1, 1997; 138(8): 3259 - 3268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cadel, T. Foulon, A. Viron, A. Balogh, S. Midol-Monnet, N. Noel, and P. Cohen Aminopeptidase B from the rat testis is a bifunctional enzyme structurally related to leukotriene-A4 hydrolase PNAS, April 1, 1997; 94(7): 2963 - 2968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Grima, L.-j. Zhu, and C. Y. Cheng Testin Is Tightly Associated with Testicular Cell Membrane upon Its Secretion by Sertoli Cells whose Steady-state mRNA Level in the Testis Correlates with the Turnover and Integrity of Inter-testicular Cell Junctions J. Biol. Chem., March 7, 1997; 272(10): 6499 - 6509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V Chesneau, A Prat, D Segretain, V Hospital, A Dupaix, T Foulon, B Jegou, and P Cohen NRD convertase: a putative processing endoprotease associated with the axoneme and the manchette in late spermatids J. Cell Sci., January 11, 1996; 109(11): 2737 - 2745. [Abstract] [PDF]
|
|
|
|
|
|
R. Blaise, T. Guillaudeux, G. Tavernier, D. Daegelen, B. Evrard, A. Mairal, C. Holm, B. Jegou, and D. Langin Testis Hormone-sensitive Lipase Expression in Spermatids Is Governed by a Short Promoter in Transgenic Mice J. Biol. Chem., February 9, 2001; 276(7): 5109 - 5115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Toure, L. Morin, C. Pineau, F. Becq, O. Dorseuil, and G. Gacon Tat1, a Novel Sulfate Transporter Specifically Expressed in Human Male Germ Cells and Potentially Linked to RhoGTPase Signaling J. Biol. Chem., June 1, 2001; 276(23): 20309 - 20315. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
