Journal of Andrology Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rune, G. M.
Right arrow Articles by Merker, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rune, G. M.
Right arrow Articles by Merker, H. J.

Journal of Andrology, Vol 13, Issue 6 560-570, Copyright © 1992 by The American Society of Andrology

JOURNAL ARTICLE

Ultrastructure of adult and juvenile marmoset (Callithrix jacchus) Sertoli cells in vivo and in vitro

G. M. Rune, D. Pretzer, P. De Souza, U. Bollmann and H. J. Merker
Department of Anatomy, Free University of Berlin, Germany.

The morphologic differentiation of Sertoli cells isolated from adult and juvenile marmosets and cultured on different extracellular matrices was evaluated by light and electron microscopy and compared to cells in vivo. Both cell types could be maintained in culture for at least 6 days. The degree of cellular differentiation, shape, ultrastructural appearance, and polarity seemed to benefit from laminin-coated substrata, compared with collagen-, fibronectin-, serum-, and heparan sulfate-coated substrata. With the former two substrates, a difference in behavior between juvenile and adult cells was evident. Whereas juvenile cells displayed a lesser degree of differentiation, adult cells exhibited identical morphologic characteristics in culture and in vivo. Cyclicity of morphologic features was not found, neither in vivo nor in vitro. The results indicate that: (1) laminin plays a unique role for marmoset Sertoli cell differentiation in vitro compared with other extracellular components; (2) a greater similarity between cells in vivo and in vitro is evident with adult Sertoli cells; and (3) the adult marmoset monkey could provide a primate model for mature Sertoli cells in culture, since there is a close similarity to human adult Sertoli cells in vitro and in vivo.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1992 by The American Society of Andrology.