Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Lamirande, E.
Right arrow Articles by Gagnon, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Lamirande, E.
Right arrow Articles by Gagnon, C.

Journal of Andrology, Vol 13, Issue 5 379-386, Copyright © 1992 by The American Society of Andrology

JOURNAL ARTICLE

Reactive oxygen species and human spermatozoa. II. Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility

E. de Lamirande and C. Gagnon
Urology Research Laboratory, Royal Victoria Hospital, Montreal, Quebec, Canada.

Under moderate conditions, reactive oxygen species (ROS) have been shown to inhibit sperm motility after several hours of incubation. The rapid decrease in flagellar beat frequency observed within the first hour of contact between ROS and spermatozoa was associated with a rapid loss of intracellular adenosine triphosphate (ATP). Motility of intact spermatozoa ceased when their ATP concentration was reduced by 85 +/- 5%. Axonemal damage was confirmed when ROS-treated spermatozoa could not reactivate motility after demembranation in a medium containing magnesium adenosine triphosphate (Mg.ATP). However, in conditions allowing rephosphorylation of the axonemes (addition of cyclic adenosine monophosphate, or cAMP, and protein kinase or sperm extracts to the demembranation medium), the motility could reactivate. Three lines of evidence suggested that ATP depletion induced by ROS treatment was responsible for the effects observed in spermatozoa. First, the rapid decrease in intracellular ATP observed after ROS treatment was closely followed by a decrease in beat frequency, loss of intact sperm motility, and axonemal damage due to insufficient phosphorylation. Second, incubation of spermatozoa with the combination pyruvate-lactate allowed maintenance of sperm ATP at a normal level and prevented the effects of ROS; furthermore, spermatozoa immobilized after ROS treatment, then supplemented with pyruvate-lactate, were able to reinitiate motility in parallel with an increase in their ATP level. Third, treatment of spermatozoa with rotenone, an ATP depleting agent, produced effects similar to ROS treatment and could also be reversed by the addition of pyruvate-lactate. These data are consistent with the conclusion that ROS treatment produced axonemal damage mostly as a result of ATP depletion.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
A. J. Koppers, G. N. De Iuliis, J. M. Finnie, E. A. McLaughlin, and R. J. Aitken
Significance of Mitochondrial Reactive Oxygen Species in the Generation of Oxidative Stress in Spermatozoa
J. Clin. Endocrinol. Metab., August 1, 2008; 93(8): 3199 - 3207.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
K. Tremellen
Oxidative stress and male infertility--a clinical perspective
Hum. Reprod. Update, May 1, 2008; 14(3): 243 - 258.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
R. A. Bronson, S. K. Bronson, and L. D. Oula
Ability of Abnormally-Shaped Human Spermatozoa to Adhere to and Penetrate Zona-Free Hamster Eggs: Correlation With Sperm Morphology and Postincubation Motility
J Androl, September 1, 2007; 28(5): 698 - 705.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
C. P. Weir and B. Robaire
Spermatozoa Have Decreased Antioxidant Enzymatic Capacity and Increased Reactive Oxygen Species Production During Aging in the Brown Norway Rat
J Androl, March 1, 2007; 28(2): 229 - 240.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
H. D. Guthrie and G. R. Welch
Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry
J Anim Sci, August 1, 2006; 84(8): 2089 - 2100.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
E. V. Zubkova and B. Robaire
Effect of Glutathione Depletion on Antioxidant Enzymes in the Epididymis, Seminal Vesicles, and Liver and on Spermatozoa Motility in the Aging Brown Norway Rat
Biol Reprod, September 1, 2004; 71(3): 1002 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
J. Baumber, B. A. Ball, J. J. Linfor, and S. A. Meyers
Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa
J Androl, July 1, 2003; 24(4): 621 - 628.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D. Perera, A. Pizzey, A. Campbell, M. Katz, J. Porter, M. Petrou, D.S. Irvine, and R. Chatterjee
Sperm DNA damage in potentially fertile homozygous {beta}-thalassaemia patients with iron overload
Hum. Reprod., July 1, 2002; 17(7): 1820 - 1825.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Aguilar-Mahecha, B. F. Hales, and B. Robaire
Chronic Cyclophosphamide Treatment Alters the Expression of Stress Response Genes in Rat Male Germ Cells
Biol Reprod, April 1, 2002; 66(4): 1024 - 1032.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Gergely, E. Kovanci, L. Senturk, E. Cosmi, L. Vigue, and G. Huszar
Morphometric assessment of mature and diminished-maturity human spermatozoa: sperm regions that reflect differences in maturity
Hum. Reprod., August 1, 1999; 14(8): 2007 - 2014.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1992 by The American Society of Andrology.