Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Buzek, S. W.
Right arrow Articles by Sanborn, B. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buzek, S. W.
Right arrow Articles by Sanborn, B. M.

Journal of Andrology, Vol 11, Issue 6 514-520, Copyright © 1990 by The American Society of Andrology

JOURNAL ARTICLE

Nuclear androgen receptor dynamics in testicular peritubular and Sertoli cells

S. W. Buzek and B. M. Sanborn
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030.

Nuclear androgen receptor dynamics were analyzed in peritubular cells and compared with those in cultured Sertoli cells. Nuclear receptors with a high affinity for [3H]dimethylnortestosterone (DMNT; mibolerone) exhibited equilibrium constants of 0.8 and 0.7 nmol, in Sertoli and peritubular cells, respectively. Time- and dose-dependent accumulation of nuclear bound receptors after exposure of whole cells to [3H]testosterone was similar for both cell types. Exogenously administered ligands demonstrated similar relative potencies as competitors with [3H]T for Sertoli and peritubular cell nuclear binding sites: DMNT greater than T greater than medroxyprogesterone acetate (MPA) greater than cyproterone acetate (CPA) tau hydroxyflutamide (OHF). Cells incubated with T or MPA showed increased nuclear androgen receptor concentrations compared to untreated controls, whereas those treated with CPA or OHF did not. These results demonstrate that the nuclear androgen receptor dynamics of peritubular cells are consistent with those of target cells. Since the dynamics are similar in Sertoli and peritubular cells, both cell types have the potential to respond to local androgen concentrations and may play important roles in androgen-dependent effects on seminiferous tubule function.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by The American Society of Andrology.