Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Warren, D. W.
Right arrow Articles by Horton, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Warren, D. W.
Right arrow Articles by Horton, R.

Journal of Andrology, Vol 11, Issue 4 353-360, Copyright © 1990 by The American Society of Andrology

JOURNAL ARTICLE

Tumor necrosis factor and interleukin-1 stimulate testosterone secretion in adult male rat Leydig cells in vitro

D. W. Warren, V. Pasupuleti, Y. Lu, B. W. Platler and R. Horton
Department of Physiology and Biophysics, University of Southern California, School of Medicine, Los Angeles 90033.

The actions of two cytokines, tumor necrosis factor (TNF) and interleukin-1 (IL-1), on testosterone production by dispersed adult testis cells and purified Leydig cells in culture were studied. In one set of experiments, testis cells from adult (90-day-old) rats were enzymatically dispersed. In another set of experiments, the dispersed testis cells were placed on a Percoll density gradient and were centrifuged to yield purified (greater than 85%) Leydig cells. Both whole testis cells and purified Leydig cells were cultured in the presence of varying doses of TNF or IL-1 with or without maximally stimulating doses of human chorionic gonadotropin (hCG). Both TNF and IL-1 stimulated basal secretion of testosterone in whole testis cells, as well as purified Leydig cells. Additionally, both TNF and IL-1 augmented maximally hCG stimulated testosterone secretion. Both cytokines stimulated testosterone secretion by dispersed testis cells as early as 4 hours, and the effect continued for up to 72 hours. The cytokines slightly, but significantly, stimulated testosterone production in purified Leydig cells after 24 hours, and continued for up to 72 hours. We have concluded from this data that TNF and IL-1 stimulate the testosterone secretion by adult rat Leydig cells. While this effect might be mediated through the action of the cytokines on testicular macrophages, there might also be a direct effect on the Leydig cell since augmentation of secretion occurred in purified Leydig cells, as well as whole testis cells. Therefore, TNF and IL-1 may serve as local regulators of Leydig cell function.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
M. O. Suescun, C. Rival, M. S. Theas, R. S. Calandra, and L. Lustig
Involvement of Tumor Necrosis Factor-{alpha} in the Pathogenesis of Autoimmune Orchitis in Rats
Biol Reprod, June 1, 2003; 68(6): 2114 - 2121.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. Zeyse, E. Lunenfeld, M. Beck, I. Prinsloo, and M. Huleihel
Induction of Interleukin-1{alpha} Production in Murine Sertoli Cells by Interleukin-1
Biol Reprod, May 1, 2000; 62(5): 1291 - 1296.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
D. Zeyse, E. Lunenfeld, M. Beck, I. Prinsloo, and M. Huleihel
Interleukin-1 Receptor Antagonist Is Produced by Sertoli Cells in Vitro
Endocrinology, April 1, 2000; 141(4): 1521 - 1527.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
L. Gnessi, A. Fabbri, and G. Spera
Gonadal Peptides as Mediators of Development and Functional Control of the Testis: An Integrated System with Hormones and Local Environment
Endocr. Rev., August 1, 1997; 18(4): 541 - 609.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by The American Society of Andrology.