Figure 10. Methylation status of hLdhc promoter CpG islands in prostate tumors (PC11, PC12, PC13, and PC14) and normal tissues (PC15). Methylation level was analyzed quantitatively by pyrosequencing. The diagram indicates a differential methylation pattern in which all 4 prostate tumor samples with various Gleason scores had a higher methylation level compared to the normal tissue control. Tumor sample PC11: Gleason grade 4 + 3, score 7, with 51%–60% tumor in the section for genomic DNA methylation analysis and no biochemical relapse. Sample PC12: Gleason grade 3 + 3, score 6, 91%–100% tumor with relapse. Sample PC13: Gleason grade 3 + 3, score 6, 90% tumor, unknown relapse. Sample PC14: Gleason grade 3 + 5, score 8, 80% tumor with relapse.