Figure 1. Western blot analysis of glycogen synthase kinase-3 (GSK-3) in bovine epididymal spermatozoa. Lane 1 shows caudal 16K supernatant immunoreactivity to an antibody against the and ß forms of GSK-3. Lane 2 uses an affinity-purified antibody against GSK-3, and lane 3 shows the immunoreactivity against GSK-3ß.